Saturday , 25 May 2019
UPDATE :
You are here: Home >> Publikasi jurnal nasional terakreditasi >> Suppression of Balb/c Lymphocyte Proliferation by Bali Cattle Testis Exctracts: The Role of TGF-?

Suppression of Balb/c Lymphocyte Proliferation by Bali Cattle Testis Exctracts: The Role of TGF-?

S.N. Depamede* & A. Rosyidi
Laboratorium Mikrobiologi dan Bioteknologi, Fakultas Peternakan, Universitas Mataram
Jln. Majapahit No. 62 Mataram NTB

ABSTRACT
Testis extract (ET) of several animals such as rats, mice, boars, and bulls, has been reported able to suppress lymphocyte proliferation. In order to elucidate if ET of bali cattle has the suppressive activity, the present study was conducted. ET was isolated aseptically from the testis of bali cattle slaughtered at a slaughter house in Mataram, Lombok. After decapsulating, the testis were minced, homogenized (1 g tissue per 3 ml phosphate buffered saline, PBS, pH 7.2). The ET (40, 20, and 10?g/ml) was added on to lymphocytes isolated from Balb/c spleen, then cultured for 72 hours in RPMI media containing 10% foetal bovine serum in 96 well plates with the concentration of 250,000 cells per well, with- or without concanavalin A (10 ?g/ml), or IL-2 (0.2 ng/ml). After 72 h of culture, cell proliferation was examined by colorimetric assay. The results showed that bali cattle ET were able to suppress the proliferation of lymphocytes in vitro through the process of apoptosis. Western blot analysis revealed that bali cattle ET contain TGF-?1. The?? suppression effects of the ET were eliminated by anti-TGF-?1 antibody. It can be concluded that ET of bali cattle expressed TGF-? which inhibited lymphocyte proliferation through an apoptotic cell mechanism. The actual roles of TGF-? in the Bali cattle testis need to be elucidated further. Key words: bali cattle testis, lymphocyte, proliferation, TGF-?1, apoptosis

DOWNLOAD File PDF

About Fahrul Irawan

Scroll To Top